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anti human cd25 pe cy7  (Cytek Biosciences)


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    Cytek Biosciences anti human cd25 pe cy7
    Anti Human Cd25 Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human cd25 pe cy7/product/Cytek Biosciences
    Average 93 stars, based on 1 article reviews
    anti human cd25 pe cy7 - by Bioz Stars, 2026-05
    93/100 stars

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    a-d, Bar charts of flow cytometry data showing expression of HLA-A2, HLA-BC, and HLA-E as percent positive expression in ( a ) hPSC-CMs, ( b ) hPSC-cFbs, ( c ) hPSC-ECs, and ( d ) hPSC-PCs by wild-type hPSC-derived cells (red) and UDET hPSC-derived cells (blue). e , Timeline of T cell cytotoxicity assay to measure T cell-mediated lysis of target cells. f-i , Percent lysis as quantified from LDH assay following exposure of activated CD8+ T cells to wild-type (red) and UDET (blue)-derived ( f ) hPSC-CMs, ( g ) hPSC-cFbs, ( h ) hPSC-ECs, and ( i ) hPSC-PCs. j , Timeline of NK cytotoxicity assay to measure NK cell-mediated lysis of target cells. k-n , Percent lysis as quantified from LDH assay following exposure of NK cells to wild-type (red) and UDET (blue)-derived ( k ) hPSC-CMs, ( l ) hPSC-cFbs, ( m ) hPSC-ECs, and ( n ) hPSC-PCs. Data shown from one donor representative of 2 biological replicates with 3-5 technical replicates. nd=no difference, *=p<0.05.

    Journal: bioRxiv

    Article Title: Hypoimmunogenic hPSC-derived cardiac organoids for immune evasion and heart repair

    doi: 10.1101/2025.04.09.648007

    Figure Lengend Snippet: a-d, Bar charts of flow cytometry data showing expression of HLA-A2, HLA-BC, and HLA-E as percent positive expression in ( a ) hPSC-CMs, ( b ) hPSC-cFbs, ( c ) hPSC-ECs, and ( d ) hPSC-PCs by wild-type hPSC-derived cells (red) and UDET hPSC-derived cells (blue). e , Timeline of T cell cytotoxicity assay to measure T cell-mediated lysis of target cells. f-i , Percent lysis as quantified from LDH assay following exposure of activated CD8+ T cells to wild-type (red) and UDET (blue)-derived ( f ) hPSC-CMs, ( g ) hPSC-cFbs, ( h ) hPSC-ECs, and ( i ) hPSC-PCs. j , Timeline of NK cytotoxicity assay to measure NK cell-mediated lysis of target cells. k-n , Percent lysis as quantified from LDH assay following exposure of NK cells to wild-type (red) and UDET (blue)-derived ( k ) hPSC-CMs, ( l ) hPSC-cFbs, ( m ) hPSC-ECs, and ( n ) hPSC-PCs. Data shown from one donor representative of 2 biological replicates with 3-5 technical replicates. nd=no difference, *=p<0.05.

    Article Snippet: Pre-conjugated antibodies used: B2M-APC (1:100; Biolegend, 316312, clone 2M2), HLA-A/B/C-PE (1:40; Biolegend, 311406, clone W6/32), HLA-E-APC (1:40; Biolegend, 342606, clone 3D12), HLA-G-APC (1:100; Biolegend, 335909, clone 87G), HLA-E-APC (1:50; Invitrogen, 17995342, clone 3D12HLA-E), HLA-BC-APC (1:50; Invitrogen, 17593542, clone B1.23.2), HLA-A2-PE-Cy7, clone BB7.2), CD25-APC (1:50; R&D Systems, FAB1020A, clone 24212), CD45-BV421 (1:50; Biolegend, 304032, clone HI30), CD3-BV605 (1:50; Biolegend, 344836, clone SK7), CD8-APC (1:50; Biolegend, 344722, clone SK1), CD4-PE (1:50; Biolegend, 980804, clone SK3).

    Techniques: Flow Cytometry, Expressing, Derivative Assay, Cytotoxicity Assay, Lysis, Lactate Dehydrogenase Assay

    Journal: bioRxiv

    Article Title: Attenuated humoral responses in HIV infection after SARS-CoV-2 vaccination are linked to global B cell defects and cellular immune profiles

    doi: 10.1101/2022.11.11.516111

    Figure Lengend Snippet:

    Article Snippet: PE-Cy7 Mouse Anti-Human CD25 , BD Biosciences , Cat # 335824 , Clone # 2A3 , 1 in 50ul.

    Techniques: Marker, Concentration Assay

    Journal: bioRxiv

    Article Title: Attenuated humoral responses in HIV infection after SARS-CoV-2 vaccination are linked to global B cell defects and cellular immune profiles

    doi: 10.1101/2022.11.11.516111

    Figure Lengend Snippet:

    Article Snippet: PE-Cy7 Mouse Anti-Human CD25 , BD Biosciences , Cat # 335824 , Clone # 2A3 , 1 in 50ul.

    Techniques: